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Wille M, etc.,al. Where do all the subtypes go? Temporal dynamics of H8-H12 influenza A viruses in waterfowl. Virus Evol. 2018 Aug 14;4(2):vey025.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Virus Evol. 2018 Aug 14;4(2):vey025 (via https://www.ncbi.nlm.nih.gov/pubmed/30151242)
Influenza A virus (IAV) is ubiquitous in waterfowl. In the northern hemisphere IAV prevalence is highest during the autumn and coincides with a peak in viral subtype diversity. Although haemagglutinin ...

Hutchinson EC, Yamauchi Y. Understanding Influenza. Methods Mol Biol. 2018;1836:1-21.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:1-21 (via https://www.ncbi.nlm.nih.gov/pubmed/30151566)
Influenza, a serious illness of humans and domesticated animals, has been studied intensively for many years. It therefore provides an example of how much we can learn from detailed studies of an infectious ...

hinson EC, Stegmann M. Purification and Proteomics of Influenza Virions. Methods Mol Biol. 2018;1836:89-120.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:89-120 (via https://www.ncbi.nlm.nih.gov/pubmed/30151570)
This chapter describes a basic workflow for analyzing the protein composition of influenza virions. In order to obtain suitable material, the chapter describes how to concentrate influenza virions from ...

Su WC, Lai MMC. Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape. Methods Mol Biol. 2018;1836:185-194.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:185-194 (via https://www.ncbi.nlm.nih.gov/pubmed/30151574)
Although several virus families are internalized into their host cells by direct fusion of the viral envelope with the plasma membrane, most viruses, for example, influenza virus, make use of endocytic ...

Chou YY, Lionnet T. Single-Molecule Sensitivity RNA FISH Analysis of Influenza Virus Genome Trafficking. Methods Mol Biol. 2018;1836:195-211.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:195-211 (via https://www.ncbi.nlm.nih.gov/pubmed/30151575)
Influenza A virus is an enveloped virus with a segmented genome consisting of eight negative-sense, single-stranded RNAs. Accumulating evidence has revealed that influenza viruses selectively package their ...

Romero-Brey I. 3D Electron Microscopy (EM) and Correlative Light Electron Microscopy (CLEM) Methods to Study Virus-Host Interactions. Methods Mol Biol. 2018;1836:213-236..  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:213-236. (via https://www.ncbi.nlm.nih.gov/pubmed/30151576)
Viruses use different strategies to interact with their host and perform a successful viral infection that results in the formation of new infectious viral particles and their propagation to new hosts. ...

Hodgson L, Verkade P, Yamauchi Y. Correlative Light and Electron Microscopy of Influenza Virus Entry and Budding. Methods Mol Biol. 2018;1836:237-260..  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:237-260. (via https://www.ncbi.nlm.nih.gov/pubmed/30151577)
Influenza A virus (IAV) entry is a stepwise process regulated by viral and cellular cues, facilitating cellular functions. Virus entry begins by attachment of hemagglutinin to cell surface sialic acids, ...

Gui L, Lee KK. Influenza Virus-Liposome Fusion Studies Using Fluorescence Dequenching and Cryo-electron Tomography. Methods Mol Biol. 2018;1836:261-279.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:261-279 (via https://www.ncbi.nlm.nih.gov/pubmed/30151578)
Influenza virus enters host cells by fusion of viral and endosomal membranes mediated by the influenza hemagglutinin (HA). The pathway of HA-catalyzed fusion has been widely investigated in influenza virus ...

Sachse M, de Castro IF, Fournier G. Metal-Tagging Transmission Electron Microscopy and Immunogold Labeling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging. Methods Mol Biol. 2018;1836:281-301.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:281-301 (via https://www.ncbi.nlm.nih.gov/pubmed/30151579)
Transmission electron microscopy (TEM) has been instrumental for studying viral infections. In particular, methods for labeling macromolecules at the ultrastructural level, by integrating biochemistry, ...

Bhagwat AR, Le Sage V, Lakdawala SS. Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy. Methods Mol Biol. 2018;1836:303-327.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:303-327 (via https://www.ncbi.nlm.nih.gov/pubmed/30151580)
Influenza viruses exhibit a complex life cycle that is still poorly understood. It involves independent replication of each of the eight segments that make up its genome and subsequent coordinated assembly ...

Miyake Y, Matthias P, Yamauchi Y. Purification of Unanchored Polyubiquitin Chains from Influenza Virions. Methods Mol Biol. 2018;1836:329-342.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:329-342 (via https://www.ncbi.nlm.nih.gov/pubmed/30151581)
Influenza A virus (IAV) is an enveloped virus with a segmented single-stranded negative-strand RNA genome. In general, the role of virally encapsidated host cell proteins in the viral life cycle is unclear. ...

Te Velthuis AJW, Long JS, Barclay WS. Assays to Measure the Activity of Influenza Virus Polymerase. Methods Mol Biol. 2018;1836:343-374.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:343-374 (via https://www.ncbi.nlm.nih.gov/pubmed/30151582)
Influenza viruses use an RNA-dependent RNA polymerase (RdRp) to transcribe and replicate their segmented negative-stranded RNA genomes. The influenza A virus RdRp consists of a heterotrimeric complex of ...

Karakus U, Crameri M, Lanz C, Yángüez E. Propagation and Titration of Influenza Viruses. Methods Mol Biol. 2018;1836:59-88..  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:59-88. (via https://www.ncbi.nlm.nih.gov/pubmed/30151569)
Influenza viruses are constantly circulating among humans, in which they cause seasonal epidemics of severe respiratory disease. Additionally, these zoonotic viruses infect different mammals and birds, ...

Anchisi S, etc.,al. Influenza A Virus Genetic Tools: From Clinical Sample to Molecular Clone. Methods Mol Biol. 2018;1836:33-58.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:33-58 (via https://www.ncbi.nlm.nih.gov/pubmed/30151568)
Implementation of reverse genetics for influenza A virus, that is, the DNA-based generation of infectious viral particles in cell culture, opened new avenues to investigate the function of viral proteins ...

Bruchhagen C, van Krüchten A, Klemm C, Ludwig S. In Vitro Models to Study Influenza Virus and Staphylococcus aureus Super-Infection on a Molecular Level. Methods Mol Biol. 2018;1836:375-386.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:375-386 (via https://www.ncbi.nlm.nih.gov/pubmed/30151583)
Investigation of pathogen-host interactions on a molecular level requires sophisticated in vitro infection procedures, especially in the presence of different pathogens.Super-infections of influenza viruses ...

Creager HM, Tumpey TM, Maines TR, Belser JA. Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus. Methods Mol Biol. 2018;1836:387-399..  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:387-399. (via https://www.ncbi.nlm.nih.gov/pubmed/30151584)
Inoculation of animals via inhaled aerosols has long been used to study the infectivity and pathogenesis of both influenza virus and other respiratory pathogens in a context that mimics natural infection. ...

Hemmink JD, Whittaker CJ, Shelton HA. Animal Models in Influenza Research. Methods Mol Biol. 2018;1836:401-430.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:401-430 (via https://www.ncbi.nlm.nih.gov/pubmed/30151585)
Animal model systems for human and animal influenza virus infection and transmission have been established to address research questions which cannot be addressed using in vitro models. Several animal ...

Karlsson EA, etc.,al. Measuring Influenza Virus Infection Using Bioluminescent Reporter Viruses for In Vivo Imaging and In Vitro Replication Assays. Methods Mol Biol. 2018;1836:431-459.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:431-459 (via https://www.ncbi.nlm.nih.gov/pubmed/30151586)
To streamline standard virological assays, we developed bioluminescent replication-competent influenza reporter viruses that mimic their parental counterparts. These reporter viruses provide a rapid and ...

Neumann G, Fan S, Kawaoka Y. Selection of Antigenically Advanced Variants of Influenza Viruses. Methods Mol Biol. 2018;1836:461-486.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:461-486 (via https://www.ncbi.nlm.nih.gov/pubmed/30151587)
Influenza virus epidemics are caused when seasonal influenza viruses (i.e., those circulating in humans) acquire mutations in the antigenic sites of the viral hemagglutinin (HA) protein that prevent the ...

Liu WC, Nachbagauer R, Krammer F, Albrecht RA. Assessment of Influenza Virus Hemagglutinin Stalk-Specific Antibody Responses. Methods Mol Biol. 2018;1836:487-511.  Abstract  
submitted by kickingbird at Aug, 31, 2018 from Methods Mol Biol. 2018;1836:487-511 (via https://www.ncbi.nlm.nih.gov/pubmed/30151588)
Animal models are essential to examine the pathogenesis and transmission of influenza viruses and for preclinical evaluation of influenza virus vaccines. Among the animal models used in influenza virus ...

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