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2019-7-24 5:09:06
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Choi WS, et al. Development of a rapid, simple and efficient one-pot cloning method for a reverse genetics system of broad subtypes of influenza A virus. Sci Rep. 2019 Jun 5;9(1):8318.  Abstract  
submitted by kickingbird at Jun, 6, 2019 from Sci Rep. 2019 Jun 5;9(1):8318 (via https://www.nature.com/articles/s41598-019-44813-z)
The reverse genetics (RG) system of influenza A viruses is well established. However, the conventional sequence-dependent method for cloning influenza genome segments is time-consuming and requires multiple ...

Scharenberg M, et al. Influenza A Virus Infection Induces Hyperresponsiveness in Human Lung Tissue-Resident and Peripheral Blood NK Cells. Front Immunol. 2019 May 17;10:1116.  Abstract  
submitted by kickingbird at Jun, 5, 2019 from Front Immunol. 2019 May 17;10:1116 (via https://www.frontiersin.org/articles/10.3389/fimmu.2019.0111)
NK cells in the human lung respond to influenza A virus- (IAV-) infected target cells. However, the detailed functional capacity of human lung and peripheral blood NK cells remains to be determined in ...

Rowe HM, et al. Direct interactions with influenza promote bacterial adherence during respiratory infections. Nat Microbiol. 2019 May 20..  Abstract  
submitted by kickingbird at Jun, 5, 2019 from Nat Microbiol. 2019 May 20. (via https://www.nature.com/articles/s41564-019-0447-0)
Epidemiological observations and animal models have long shown synergy between influenza virus infections and bacterial infections. Influenza virus infection leads to an increase in both the susceptibility ...

Gong S, et al. Human-Derived A/Guangdong/Th005/2017 (H7N9) Exhibits Extremely High Replication in the Lungs of Ferrets and Is Highly Pathogenic in Chickens. Viruses. 2019 May 29;11(6.  Abstract  
submitted by kickingbird at Jun, 3, 2019 from Viruses. 2019 May 29;11(6 (via https://www.mdpi.com/1999-4915/11/6/494)
After a series of studies on the pathogenicity of several H7N9 strains from 2013 to 2018, we wanted to dynamically track the pathogenicity of A/Guangdong/Th005/2017 in ferrets and poultry. The pathogenicity ...

Antigua KJC, Choi WS, Baek YH, Song MS. The Emergence and Decennary Distribution of Clade 2.3.4.4 HPAI H5Nx. Microorganisms. 2019 May 29;7(6)..  Abstract  
submitted by kickingbird at Jun, 3, 2019 from Microorganisms. 2019 May 29;7(6). (via https://www.mdpi.com/2076-2607/7/6/156)
Reassortment events among influenza viruses occur naturally and may lead to the development of new and different subtypes which often ignite the possibility of an influenza outbreak. Between 2008 and 2010, ...

Levy ER, et al. Evaluation of Mannose Binding Lectin Gene Variants in Pediatric Influenza Virus-Related Critical Illness. Front Immunol. 2019 May 8;10:1005..  Abstract  
submitted by kickingbird at May, 31, 2019 from Front Immunol. 2019 May 8;10:1005. (via https://www.frontiersin.org/articles/10.3389/fimmu.2019.0100)
Background: Mannose-binding lectin (MBL) is an innate immune protein with strong biologic plausibility for protecting against influenza virus-related sepsis and bacterial co-infection. In an autopsy cohort ...

Du R, Cui Q, Rong L. Competitive Cooperation of Hemagglutinin and Neuraminidase during Influenza A Virus Entry. Viruses. 2019 May 20;11(5)..  Abstract  
submitted by kickingbird at May, 31, 2019 from Viruses. 2019 May 20;11(5). (via https://www.mdpi.com/1999-4915/11/5/458)
The hemagglutinin (HA) and neuraminidase (NA) of influenza A virus possess antagonistic activities on interaction with sialic acid (SA), which is the receptor for virus attachment. HA binds SA through ...

Wu et al.. Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational Incompatibility. Cell Host & Microbe.  Abstract  
submitted by kickingbird at May, 29, 2019 from Cell Host & Microbe (via https://www.cell.com/cell-host-microbe/fulltext/S1931-3128(1)
Egg-based seasonal influenza vaccines are the major preventive countermeasure against influenza virus. However, their effectiveness can be compromised when antigenic changes arise from egg-adaptive mutations ...

Nelson SA, Sant AJ. Imprinting and Editing of the Human CD4 T Cell Response to Influenza Virus. Front Immunol. 2019 May 7;10:932..  Abstract  
submitted by kickingbird at May, 29, 2019 from Front Immunol. 2019 May 7;10:932. (via https://www.frontiersin.org/articles/10.3389/fimmu.2019.0093)
Immunity to influenza is unique among pathogens, in that immune memory is established both via intermittent lung localized infections with highly variable influenza virus strains and by intramuscular vaccinations ...

Farrukee R, Butler J, Reading PC, Hurt AC. Characterization of substitutions in the neuraminidase of A(H7N9) influenza viruses selected following serial passage in the presence of different neuraminidase inhibitors. Antiviral Res. 2019 May 24..  Abstract  
submitted by kickingbird at May, 29, 2019 from Antiviral Res. 2019 May 24. (via https://www.sciencedirect.com/science/article/pii/S016635421)
Avian A(H7N9) infections in humans have been reported in China since 2013 and are of public health concern due to their severity and pandemic potential. Oseltamivir and peramivir are neuraminidase inhibitors ...

Spackman E, et al. Assessment of replicate numbers for titrating avian influenza virus using dose-response models. J Vet Diagn Invest. 2019 May 25:1040638719853851..  Abstract  
submitted by kickingbird at May, 29, 2019 from J Vet Diagn Invest. 2019 May 25:1040638719853851. (via https://www.ncbi.nlm.nih.gov/pubmed/31131714)
Embryonating chicken eggs (ECEs) are among the most sensitive laboratory host systems for avian influenza virus (AIV) titration, but ECEs are expensive and require space for storage and incubation. Therefore, ...

Guinat C, Rouchy N, Camy F, Guérin JL, Paul MC. Exploring the Wind-Borne Spread of Highly Pathogenic Avian Influenza H5N8 During the 2016-2017 Epizootic in France. Avian Dis. 2018 Oct 11;63.  Abstract  
submitted by kickingbird at May, 29, 2019 from Avian Dis. 2018 Oct 11;63 (via https://bioone.org/journals/Avian-Diseases/volume-63/issue-s)
In winter 2016-2017, highly pathogenic avian influenza (HPAI) H5N8 virus spread in France, causing an unprecedented epizootic. During the epidemic, southwest France, where most outbreaks were reported, ...

Pénzes Z, et al. Rapid Construction and Immunogenicity Testing of a Novel H5 Virus-Like Particle Prototype Vaccine Against Clade 2.3.4.4 H5N8 Highly Pathogenic Avian Influenza Virus. Avian Dis. 2018 Dec 18;63.  Abstract  
submitted by kickingbird at May, 29, 2019 from Avian Dis. 2018 Dec 18;63 (via https://bioone.org/journals/Avian-Diseases/volume-63/issue-s)
From October 2016 to July 2017, 47 countries have been affected by highly pathogenic avian influenza (HPAI) viruses of the H5N8 clade 2.3.4.4 subtype, including European and African, and it has been the ...

Reid SM, et al. Two Single Incursions of H7N7 and H5N1 Low Pathogenicity Avian Influenza in U.K. Broiler Breeders During 2015 and 2016. Avian Dis. 2018 Dec 17;63.  Abstract  
submitted by kickingbird at May, 29, 2019 from Avian Dis. 2018 Dec 17;63 (via https://bioone.org/journals/Avian-Diseases/volume-63/issue-s)
Low pathogenicity (LP) avian influenza viruses (AIVs) have a natural reservoir in wild birds. These cause few (if any) overt clinical signs, but include H5 and H7 LPAIVs, which are notifiable in poultry. ...

Jagadesh A, et al. Genetic characterization of hemagglutinin (HA) gene of influenza A viruses circulating in Southwest India during 2017 season. Virus Genes. 2019 May 25.  Abstract  
submitted by kickingbird at May, 29, 2019 from Virus Genes. 2019 May 25 (via https://link.springer.com/article/10.1007%2Fs11262-019-01675)
Molecular surveillance of influenza viruses is essential for early detection of novel variants. The aim of the present study was to analyze the hemagglutinin gene of influenza A(H1N1)pdm09 and A(H3N2) ...

Zhang H, et al. Development of a multiplex real-time RT-PCR assay for simultaneous detection and differentiation of influenza A, B, C, and D viruses. Diagn Microbiol Infect Dis. 2019 Apr 29..  Abstract  
submitted by kickingbird at May, 29, 2019 from Diagn Microbiol Infect Dis. 2019 Apr 29. (via https://www.ncbi.nlm.nih.gov/pubmed/31130238)
Influenza is a common and contagious respiratory disease caused by influenza A, B, C, and D viruses (IAV, IBV, ICV, and IDV). A multiplex real-time RT-PCR assay was developed for simultaneous detection ...

Hu B, H?fer CT, Thiele C, Veit M. Cholesterol binding to the transmembrane region of a group 2 HA of Influenza virus is essential for virus replication affecting both virus assembly and HA´s fusion activity. J Virol. 2019 May 22..  Abstract  
submitted by kickingbird at May, 26, 2019 from J Virol. 2019 May 22. (via https://www.ncbi.nlm.nih.gov/pubmed/31118253)
Hemagglutinin (HA) of Influenza virus is incorporated into cholesterol enriched, nanodomains of the plasma membrane. Phylogenetic group 2 HAs contain the conserved cholesterol consensus motif (CCM) YKLW ...

Gilbert PB, et al. HAI and NAI titer correlates of inactivated and live attenuated influenza vaccine efficacy. BMC Infect Dis. 2019 May 22;19(1):453.  Abstract  
submitted by kickingbird at May, 26, 2019 from BMC Infect Dis. 2019 May 22;19(1):453 (via https://bmcinfectdis.biomedcentral.com/articles/10.1186/s128)
BACKGROUND: High hemagglutination inhibition (HAI) and neuraminidase inhibition (NAI) titers are generally associated with reduced influenza risk. While repeated influenza vaccination reduces seroresponse, ...

Zhang Y, et al. Genetic and biological characteristics of avian influenza virus subtype H1N8 in environments related to live poultry markets in China. BMC Infect Dis. 2019 May 22;19(1):458.  Abstract  
submitted by kickingbird at May, 26, 2019 from BMC Infect Dis. 2019 May 22;19(1):458 (via https://bmcinfectdis.biomedcentral.com/articles/10.1186/s128)
BACKGROUND: Since 2008, avian influenza surveillance in poultry-related environments has been conducted annually in China. Samples have been collected from environments including live poultry markets, ...

Yu Z, et al. PB2 and hemagglutinin mutations confer a virulent phenotype on an H1N2 avian influenza virus in mice. Arch Virol. 2019 May 20.  Abstract  
submitted by kickingbird at May, 23, 2019 from Arch Virol. 2019 May 20 (via https://link.springer.com/article/10.1007%2Fs00705-019-04283)
We previously obtained mouse-adapted variants of H1N2 avian influenza virus that contained PB2-L134H, PB2-I647L, PB2-D701N, HA-G228S, and M1-D231N mutations. Here, we analyzed the effects of these mutations ...

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