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2025-4-1 2:24:34
Article

Oliver Eales, James M McCaw, Freya M Shearer. [preprint]Viral kinetics of H5N1 infections in dairy cattle. https://doi.org/10.1101/2025.02.01.636082.  Abstract  
submitted by kickingbird at Feb, 6, 2025 from https://doi.org/10.1101/2025.02.01.636082 (via https://www.biorxiv.org/content/10.1101/2025.02.01.636082v1)
Since early-2024 unprecedented outbreaks of highly pathogenic avian influenza H5N1 clade 2.3.4.4b have been ongoing in dairy cattle in the United States with significant consequences for the dairy industry ...

Weibo Tang, etc.,al. Rapid aging of influenza epidemics in China from 2005/06 to 2016/17: a population-based study. Infectious Disease Modelling.  Abstract  
submitted by kickingbird at Feb, 6, 2025 from Infectious Disease Modelling (via https://www.sciencedirect.com/science/article/pii/S246804272)
BackgroundChina is an aging society, and the older population is at a higher risk of influenza infection and influenza-related mortality. However, there is limited knowledge regarding the aging of influenza ...

Mahmoud SH, Khattab MS, Yehia N, Zanaty A, Arafa A. Pathogenicity of Highly Pathogenic Avian Influenza A/H5Nx Viruses in Avian and Murine Models. Pathogens. 2025; 14(2):149.  Abstract  
submitted by kickingbird at Feb, 5, 2025 from Pathogens. 2025; 14(2):149 (via https://www.mdpi.com/2076-0817/14/2/149)
The evolution and adaptation of highly pathogenic avian influenza (HPAI) viruses pose ongoing challenges for animal and public health. We investigated the pathogenic characteristics of the newly emerged ...

Hannah L. Wallace, etc.,al. [preprint]No Evidence of Anti-influenza Nucleoprotein Antibodies in Retail Milk from Across Canada (April - July 2024). https://doi.org/10.1101/2025.01.31.25321461.  Abstract  
submitted by kickingbird at Feb, 5, 2025 from https://doi.org/10.1101/2025.01.31.25321461 (via https://www.medrxiv.org/content/10.1101/2025.01.31.25321461v)
Following reports of HPAI H5N1 infections of dairy cattle in the United States (US) in March 2024, we established a Pan-Canadian Milk network to monitor retail milk in Canada. Milk samples from across ...

Artois J, Vergne T, Fourtune L, Dellicour S, Scoiz. Spatial risk modelling of highly pathogenic avian influenza in France: Fattening duck farm activity matters. PLoS One. 2025 Feb 4;20(2):e0316248.  Abstract  
submitted by kickingbird at Feb, 5, 2025 from PLoS One. 2025 Feb 4;20(2):e0316248 (via https://journals.plos.org/plosone/article?id=10.1371/journal)
In this study, we present a comprehensive analysis of the key spatial risk factors and predictive risk maps for HPAI infection in France, with a focus on the 2016-17 and 2020-21 epidemic waves. Our findings ...

Hirst JC, Hutchinson EC. Purification of Influenza Virions. Methods Mol Biol. 2025;2890:27-51.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Methods Mol Biol. 2025;2890:27-51 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
This chapter describes basic workflows for concentrating and for purifying influenza virions. It presents several ways in which ultracentrifugation can be used to concentrate influenza virions from the ...

Zhou J, Hemmink JD, Whittaker CJ, Shelton HA, Peac. Animal Models in Influenza Research. Methods Mol Biol. 2025;2890:53-88.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Methods Mol Biol. 2025;2890:53-88 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Animal model systems for human and animal influenza virus infection and transmission have been established to address research questions which cannot be addressed using in vitro models. Several animal ...

Broich L, Fu Y, Sieben C. Live-Cell Single-Molecule Imaging of Influenza A Virus-Receptor Interaction. Methods Mol Biol. 2025;2890:89-101.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Methods Mol Biol. 2025;2890:89-101 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Influenza A viruses are a major health care burden, and their biology has been intensely studied for decades. However, many details of virus infection are still elusive, requiring the development of refined ...

Pathak T, Gupta K, Kumar N, Banerjee I. Monitoring Influenza A Virus Entry Using Quantitative Fluorescence Microscopy. Methods Mol Biol. 2025;2890:103-123.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Methods Mol Biol. 2025;2890:103-123 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Influenza A virus (IAV) is a major threat to global human health and is a topic of intense research. With the continuous problem of seasonal influenza and the threat of potential pandemics due to frequent ...

Nakano M, Noda T. Purification and Ultramicroscopic Observation of the Influenza A Virus Ribonucleoprotein Complex. Methods Mol Biol. 2025;2890:141-149.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Methods Mol Biol. 2025;2890:141-149 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Influenza A virus (IAV) has an eight-segmented, single-stranded, negative-sense viral genomic RNA (vRNA). Each vRNA strand associates with nucleoproteins and an RNA-dependent RNA polymerase complex to ...

Peterl S, Wachsmuth-Melm M, Chlanda P. Sample Preparation for Cryo-Electron Tomography of Influenza A Virus and Infected Cells. Methods Mol Biol. 2025;2890:169-184.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Methods Mol Biol. 2025;2890:169-184 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Influenza A virus (IAV) replication within host cells relies on tightly controlled, dynamic interactions between viral and cellular components. Yet we have a limited understanding of the viral replication ...

Sousa AL, Tranfield EM. Room Temperature Transmission Electron Microscopy of Influenza A Virus Samples. Methods Mol Biol. 2025;2890:151-167.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Methods Mol Biol. 2025;2890:151-167 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Transmission electron microscopy is an imaging technique that visualizes the ultrastructure of samples, revealing not just the structures of interest but also the context occurring around the structures ...

Lee N. Mapping Binding Sites of Nucleoprotein Within the Influenza Virus RNA Genome. Methods Mol Biol. 2025;2890:211-224.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Methods Mol Biol. 2025;2890:211-224 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
With the advent of next-generation sequencing, a plethora of techniques have been developed to uncover nucleic acid interactions with unprecedented resolution. For example, UV-crosslinking and immunoprecipitation ...

Cha RM, Park MJ, Baek YG, Lee YN, Jang Y, Kang YM,. Genetic characteristics and pathogenesis of clade 2.3.4.4b H5N1 high pathogenicity avian influenza virus isolated from poultry in South Korea, 2022-2023. Virus Res. 2025 Jan 31:199541.  Abstract  
submitted by kickingbird at Feb, 3, 2025 from Virus Res. 2025 Jan 31:199541 (via https://pubmed.ncbi.nlm.nih.gov/39894372/)
During the 2022-2023 winter season in South Korea, a novel clade 2.3.4.4b H5N1 HPAIV was first detected in wild birds, which then subsequently caused multiple outbreaks in poultry farms and wild birds. ...

Kirkpatrick Roubidoux E, Meliopoulos V, Livingston. Intraductal infection with H5N1 clade 2.3.4.4b influenza virus. J Virol. 2025 Jan 31:e0192724.  Abstract  
submitted by kickingbird at Feb, 1, 2025 from J Virol. 2025 Jan 31:e0192724 (via https://journals.asm.org/doi/10.1128/jvi.01927-24)
In March 2024, highly pathogenic avian influenza (HPAI) H5N1 of the clade 2.3.4.4b was detected in dairy cows in Texas and has since been detected in several other U.S. states. Virus has been detected ...

Beate M. Crossley, etc.,al. In laboratory inactivation of H5N1 in raw whole milk through milk acidification: results from a pilot study. Journal of Dairy Science.  Abstract  
submitted by kickingbird at Jan, 30, 2025 from Journal of Dairy Science (via https://www.sciencedirect.com/science/article/pii/S002203022)
Avian Influenza virus H5N1 2.3.4.4.b has recently been detected in cattle, with milk from infected animals reported to contain a high viral load, serving as a potential source for shedding and dissemination ...

Nicholas C. Morano, etc.,al. Structure of a zoonotic H5N1 hemagglutinin reveals a receptor-binding site occupied by an auto-glycan. Structure, January 2025.  Abstract  
submitted by kickingbird at Jan, 30, 2025 from Structure, January 2025 (via https://www.sciencedirect.com/science/article/abs/pii/S09692)
Highly pathogenic avian influenza has spilled into many mammals, most notably cows and poultry, with several dozen human breakthrough infections. Zoonotic crossovers, with hemagglutinins mutated to enhance ...

Huarui Duan, etc.,al. Computational design and improvement of a broad influenza virus HA stem targeting antibody. Structure, January 2025.  Abstract  
submitted by kickingbird at Jan, 30, 2025 from Structure, January 2025 (via https://www.sciencedirect.com/science/article/abs/pii/S09692)
Broadly neutralizing antibodies (nAbs) are vital therapeutic tools to counteract both pandemic and seasonal influenza threats. Traditional strategies for optimizing nAbs generally rely on labor-intensive, ...

Nishiyama A, Nogimori T, Masuta Y, Matsuura T, Kas. Cross-Reactive Fc-Mediated Antibody Responses to Influenza HA Stem Region in Human Sera Following Seasonal Vaccination. Vaccines. 2025; 13(2):140.  Abstract  
submitted by kickingbird at Jan, 30, 2025 from Vaccines. 2025; 13(2):140 (via https://www.mdpi.com/2076-393X/13/2/140)
Background: Current influenza A vaccines primarily induce neutralizing antibodies targeting the variable hemagglutinin (HA) head domain, limiting their effectiveness against diverse or emerging influenza ...

Thomas Rawson, etc.,al. [preprint]A mathematical model of H5N1 influenza transmission in US dairy cattle.. https://doi.org/10.1101/2025.01.28.25321250.  Abstract  
submitted by kickingbird at Jan, 30, 2025 from https://doi.org/10.1101/2025.01.28.25321250 (via https://www.medrxiv.org/content/10.1101/2025.01.28.25321250v)
We present a stochastic metapopulation transmission model that simulates the spread of H5N1 avian influenza through individual dairy cows in 35,974 dairy herds in the continental United States. Transmission ...

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