Varich NL, Sadykova GK, Prilipov AG, Kochergin-Nik. Location and architecture of an antibody-binding site of influenza A virus nucleoprotein. Arch Virol 2013
Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutagenesis and ELISA to screen the amino acids surrounding position 470 recognized by the MAb 3/1 as a linear epitope. Ten amino acid residues involved in the reaction of NP with the MAb 3/1 and the MAb 469/6 were identified. Our data are the first to outline a compact site recognized by MAbs in the 3D structure of the influenza virus NP.
See Also:
Latest articles in those days:
- Mapping Genetic Markers Associated with Antigenicity and Host Range in H9N2 Influenza A Viruses Infecting Poultry in Pakistan 19 hours ago
- Wastewater-based surveillance is an efficient monitoring tool for tracking influenza A in the community 19 hours ago
- Iceland: an underestimated hub for the spread of high-pathogenicity avian influenza viruses in the North Atlantic 19 hours ago
- Highly Pathogenic Avian Influenza A(H5N1) Virus Infection in a Dairy Farm Worker 1 days ago
- [preprint] Emergence and interstate spread of highly pathogenic avian influenza A(H5N1) in dairy cattle 2 days ago
[Go Top] [Close Window]