Lai JC, Chan WW, Kien F, Nicholls JM, Peiris JS, G. Formation of virus-like particles from human cell lines exclusively expressing Influenza neuraminidase. J Gen Virol. 2010 May 26
The minimal viral requirements for the generation of influenza virus like particle (VLP) assembly and budding was reassessed. Using neuraminidase from the H5N1 and H1N1 subtypes, it was found that the expression of neuraminidase (NA) alone was sufficient to generate and release VLPs. Biochemical and functional characterization of the NA containing VLPs demonstrated that they were morphologically similar to influenza virions. The NA oligomerization was comparable to that of the live virus, and the enzymatic activity, while not required for the release of NA-VLP, was preserved. Together, these findings indicate that NA plays a key role in virus budding and morphogenesis and demonstrates that NA-VLPs represent a useful tool in influenza research.
See Also:
Latest articles in those days:
- The evolution, complexity, and diversity of swine influenza viruses in China: A hidden public health threat 1 days ago
- MHC class II proteins mediate sialic acid independent entry of human and avian H2N2 influenza A viruses 1 days ago
- Histopathologic Features and Viral Antigen Distribution of H5N1 Highly Pathogenic Avian Influenza Virus Clade 2.3.4.4b from the 2022–2023 Outbreak in Iowa Wild Birds 1 days ago
- Detection and characterization of H5N1 HPAIV in environmental samples from a dairy farm 1 days ago
- Genomic Characterization of Highly Pathogenic Avian Influenza A H5N1 Virus Newly Emerged in Dairy Cattle 1 days ago
[Go Top] [Close Window]