Song L, Nakaar V, Kavita U, Price A, Huleatt J, Ta. Efficacious recombinant influenza vaccines produced by high yield bacterial expression: a solution to global pandemic and seasonal needs. PLoS ONE. 2008 May 21;3(5):e2257
It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. We have used this approach to generate novel influenza vaccines that fuse the globular head domain of the protective hemagglutinin (HA) antigen with the potent TLR5 ligand, flagellin. These fusion proteins are efficiently expressed in standard E. coli fermentation systems and the HA moiety can be faithfully refolded to take on the native conformation of the globular head. In mouse models of influenza infection, the vaccines elicit robust antibody responses that mitigate disease and protect mice from lethal challenge. These immunologically potent vaccines can be efficiently manufactured to support pandemic response, pre-pandemic and seasonal vaccines.
- Serological Evidence of Influenza D Virus Circulation Among Cattle and Small Ruminants in France 15 hours ago
- Comparison of Avian Influenza Virus Contamination in the Environment Before and After Massive Poultry H5/H7 Vaccination in Zhejiang Province, China 15 hours ago
- Adaptive amino acid substitutions enhance the virulence of an avian-origin H6N1 influenza virus in mice 15 hours ago
- Emergence of waterfowl-originated gene cassettes in HPAI H7N9 viruses caused severe human infection in Fujian, China 3 days ago
- Detection of oseltamivir-resistant zoonotic and animal influenza A viruses using the rapid influenza antiviral resistance test 3 days ago