Objective To establish a specific, sensitive, rapid method to detect virus gene of H5 sub- type inactivated vaccine and to analyze the correlation between the gene copy number and the HI antibody titer. Methods Specific primers and TaqMan MGB(minor groove binder) probes were designed with Primer Express 2.0 software according to the conserved region of the H5 subtype AIV hemagglutinin (HA) gene to make research of real-time fluorescent PCR in the quantitative detection of H5 subtype avian influenza inactiva...

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