HAN Jian-feng; NING Yi-bao; JIANG Tao-zhen; LI Nin. Rapid quantitative detection of H5 subtype avian influenza inactivated vaccine by real-time fluorescent PCR and exploration of vaccine efficacy by in vitro test. Chinese Journal of Microbiology and Immunology
Objective To establish a specific, sensitive, rapid method to detect virus gene of H5 sub- type inactivated vaccine and to analyze the correlation between the gene copy number and the HI antibody titer. Methods Specific primers and TaqMan MGB(minor groove binder) probes were designed with Primer Express 2.0 software according to the conserved region of the H5 subtype AIV hemagglutinin (HA) gene to make research of real-time fluorescent PCR in the quantitative detection of H5 subtype avian influenza inactiva...
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