HAN Qing-gong; QIAO Chuan-ling etc.,al. Sequencing and eukaryotic expression of HA gene of H9N2 subtype influenza virus from swine. Chinese Journal of Preventive Veterinary Medicine
HA gene of SW/ZhJ7/04 was amplified by RT-PCR,sequenced,and cloned into recombinant plasmid pCAGGS-HA. The 293T cells monolayer were transfected with plasmid pCAGGS-HA,expression of the HA protein was detected by SDS-PAGE 48 h after transfection and its immunogenic activity was confirmed by Western blot assay.The immunogenicity of the plasmid pCAGGS-HA was evaluated in mice.
The Chinese version of fulltext can be downloaded at Chinese version of F.I.C
See Also:
Latest articles in those days:
- Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation 2 days ago
- Risk assessment of a highly pathogenic H5N1 influenza virus from mink 2 days ago
- Detection of clade 2.3.4.4b highly pathogenic H5N1 influenza virus in New York City 2 days ago
- Sequence-based epitope mapping of high pathogenicity avian influenza H5 clade 2.3.4.4b in Latin America 3 days ago
- Guanylate-binding protein 1 inhibits inflammatory factors produced by H5N1 virus through Its GTPase activity 3 days ago
[Go Top] [Close Window]