In this study,the HPAIV A/Tiger/Harbin/01/2002(H5N1) used was originated from tigers and propagated in SPF embryonated hen eggs.TCID50 of the virus was 10-7.36/0.05mL on MDCK cell.The cats were inoculated through bronchus route and then,the cats of dead and control were collected for histopathological and immunohistochemistry examination.Meanwhile,the emulsion supernatant fluid of organs and the pharyngeal swab samples of the dead cats were collected for RT-PCR,survived cats and the control cats were tested...

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