Ng SS, Li OT, Cheung TK, Peiris JM, Poon LL. Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans. Virol J. 2008 Jan 11;5(1):2
ABSTRACT: The initiation of transcription and replication of influenza A virus requires the 5´ and 3´ ends of vRNA. Here, the role of segment-specific non-coding sequences of influenza A virus on viral RNA synthesis was studied. Recombinant viruses, with the nonstructural protein (NS) segment-specific non-coding sequences replaced by the corresponding sequences of the neuraminidase (NA) segment, were characterized. The NS and NA vRNA levels in cells infected with these mutants were much higher than those of the wild type, whereas the NS and NA mRNA levels of the mutants were comparable to the wild-type levels. By contrast, the PB2 vRNA and mRNA levels of all the tested viruses were similar, indicating that vRNA with heterologous segment-specific non-coding sequences was not affected by the mutations. The observations suggested that, with the cooperation between the homologous 5´ and 3´segment-specific sequences, the introduced mutations could specifically enhance the replication of NA and NS vRNA.
See Also:
Latest articles in those days:
- Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation 21 hours ago
- Risk assessment of a highly pathogenic H5N1 influenza virus from mink 21 hours ago
- Detection of clade 2.3.4.4b highly pathogenic H5N1 influenza virus in New York City 21 hours ago
- Sequence-based epitope mapping of high pathogenicity avian influenza H5 clade 2.3.4.4b in Latin America 2 days ago
- Guanylate-binding protein 1 inhibits inflammatory factors produced by H5N1 virus through Its GTPase activity 2 days ago
[Go Top] [Close Window]