LIU Bin; LI Wen-ping; QU Xiao-chu; TANG Yu-long; Z. Expression of nucleoprotein of avian influenza virus bait vectors and detection of its self-activation in yeast two-hybrid system. Chinese Journal of Zoonoses
To express of nucleoprotein(NP) of avian influenza virus(H5N1) in yeast two-hybrid system and to detect its self-activation in this system,the complete encoding gene of NP was amplified from a plasmid pGEMT/H5NP by PCR and it was inserted into pGBKT7 vector according to the reading frame.The bait vectors were transformed into the yeast strain AH109 by PEG/LiAc method and its self-activation was tested by both the phenotype assay and the color assay,then expression of Gal4-NP fusion proteins identified by We...
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