Abstract: Hemagglutin inInhibition test is used in this study to monitor the 54 serum samples from healthy hawks, pigeons and quails in the eco system in Hubei and Guangxi province, where there were outbreaks of H5 subtype HPAI in 2004. In addition, the data are statistically analyzed. The results show that the positive rates of anti, H9 subtype, H6 subtype aiv and H5 subtype aiv antibodies is 64. 8%、75. 9% and 29.6% respectively. According to these results, we drew the conclusion that H9 sub type aiv, H6 sub type aiv and H5 sub type aiv circulated around this eco system. And H9 subtype aiv and H6 subtype aiv circulated stably and chronically in this eco system and are the leading prevalent serotype. On the other hand, the positive
rate of anti H5 subtype aiv antibodies is 29. 6% , the highest titer of antibodies of that is 640 ×. It is impossible that the H5 subtype aiv is newly transmitted into the eco system or a new recombinant strain of virus. And it is correlated to the outbreaks at this time there, which need to be validated at a molecular level.
Key words: eco system; antibody anti aiv; Hemagglutinin Inhibition test; statistics

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