Wang S, Taaffe J, Parker C, Solorzano A, Cao H, Ga. The Hemagglutinin (HA) Proteins from H1 and H3 Serotypes of Influenza A Viruses Require Different Antigen Designs for the Induction of Optimal Protective Antibody Responses as Studied by Codon Optimiz. J Virol. 2006 Sep 20; [Epub ahead of print]
Effective antibody responses provide crucial immunity against influenza (Flu) virus infection. The hemagglutinin (HA) protein is the major target of protective antibody responses induced by viral infection and by vaccination with both inactivated and live attenuated Flu vaccines but the knowledge on the optimal designs of protective HA antigens from different Flu serotypes is still limited. In this study, we have significantly improved the immunogenicity of HA expressing DNA vaccines by using codon optimized HA sequences for either an H1 serotype (A/NewCal/20/99) or an H3 serotype (A/Panama/2007/99) human influenza A virus, and then used these constructs as model antigens to identify the optimal HA antigen designs to elicit high level protective antibody responses. Two forms of HA antigen, a wild type full length HA and a secreted form with transmembrane (TM) domain truncated HA, were produced. Both forms of HA DNA vaccines, either from H1 or H3 serotypes, were able to elicit high levels of HA-specific IgG responses, in immunized rabbits as measured by ELISA. Interestingly, their abilities in eliciting hemagglutination inhibition (HI) and neutralizing antibody (NAb) responses differ. For the H1 HA antigens, the full length HA induced significantly higher HI and NAb responses than the TM truncated HA. For the H3 HA antigen, both the full length HA and TM truncated HA induced high levels of HI and NAb responses. These data indicate that H1 and H3 antigens have different expression requirements for the induction of an optimal protective antibody response and that the structure integrity of HA antigens is critical in eliciting type specific protective antibody responses. Our findings would have an important impact to future subunit based Flu vaccine development.
See Also:
Latest articles in those days:
- The evolution, complexity, and diversity of swine influenza viruses in China: A hidden public health threat 17 hours ago
- MHC class II proteins mediate sialic acid independent entry of human and avian H2N2 influenza A viruses 17 hours ago
- Histopathologic Features and Viral Antigen Distribution of H5N1 Highly Pathogenic Avian Influenza Virus Clade 2.3.4.4b from the 2022–2023 Outbreak in Iowa Wild Birds 17 hours ago
- Detection and characterization of H5N1 HPAIV in environmental samples from a dairy farm 21 hours ago
- Genomic Characterization of Highly Pathogenic Avian Influenza A H5N1 Virus Newly Emerged in Dairy Cattle 21 hours ago
[Go Top] [Close Window]