H5N1 highly pathogenic avian influenza virus was highly pathogenic and sometimes even fatal for tigers and cats.To develop a new type of vaccine for Felidae influenza prevention,recombinant replication-competent canine adenovirus Type 2 expressing hemagglutinin gene of H5N1 subtype tiger influenza virus was constructed.A/tiger/Harbin/01/2003(H5N1)HA gene was cloned into PVAX1.The HA expression cassette which included CMV and HA and PolyA was ligated into the E3 deletion region of pVAXΔE.The recombinant plas...

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