CHANG S, ZHENG XY, YANG ST, ZOU XT, Ding Z, XIA XZ. Determining the Distribution and Contents of High Pathogenic Avian influenza virus from Tiger in Mice. Progress in Veterinary Medicine 2006 05
With the dilution of 10~-3 from the third generation of allantoic fluid in embryonated eggs infected by H5N1 high pathogenic avian influenza virus A/Tiger/Harbin/01/2003 strain which was isolated and verified in this laboratory,mice were inoculated.After mouse death,we collected hearts,livers,spleens,lungs,kidneys and brains to prepare emulsion.And meanwhile,MDCK cells were inoculated to observe the cytopathogenic effect as the determinant index.The contents of virus in each organ of dead mouse was calculat...
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