LIU Guang-liang, etc.,al. Construction and transient expression of recombinant avian influenza virus with nucleoprotein gene. Chinese Journal of Veterinary Science and Tech
The NP gene of the AIV A/Goose/Guangdong/1/96(H5N1) strain was amplified from a recombinant plasmid pMel-NP by PCR and subcloned into an eukaryotic expression vector pVAX1 and the recombinant vector was designated pVAX-NP.The high quality pVAX-NP was prepared and the Hela cells were transfected.The recombinant vector pVAX-NP was successfully expressed in the cells.Indirect immunofluorescence assay and Western-blotting analysis showed that the nucleoprotein was expressed transiently in the Hela cells,which w...
See Also:
Latest articles in those days:
- The evolution, complexity, and diversity of swine influenza viruses in China: A hidden public health threat 17 hours ago
- MHC class II proteins mediate sialic acid independent entry of human and avian H2N2 influenza A viruses 17 hours ago
- Histopathologic Features and Viral Antigen Distribution of H5N1 Highly Pathogenic Avian Influenza Virus Clade 2.3.4.4b from the 2022–2023 Outbreak in Iowa Wild Birds 17 hours ago
- Detection and characterization of H5N1 HPAIV in environmental samples from a dairy farm 21 hours ago
- Genomic Characterization of Highly Pathogenic Avian Influenza A H5N1 Virus Newly Emerged in Dairy Cattle 21 hours ago
[Go Top] [Close Window]