Highly pathogenic avian influenza (HPAI) H5N1 clade 2.3.4.4b genotype B3.13 was confirmed in a dairy cow in Texas on March 25, 2024, by the US Department of Agriculture (USDA) National Veterinary Services Laboratories (NVSL) in response to a multi-state investigation into milk production losses. The amount and duration of virus shed in milk from the inoculated mammary quarters point to milk as a primary source of virus spread within and between dairy herds. Pasteurization has been shown to inactive the virus in milk, however, domestic cats consuming raw milk from affected cows developed fatal systemic influenza infection. Investigations on affected dairy herds demonstrated the presence of viral RNA in milk samples for up to 2 weeks before the appearance of clinical signs and diagnostic confirmation of infection. During this lag, unpasteurized milk may pose a risk to calves. Here, we sought to determine if H5N1 genotype B3.13 strain could be transmitted to calves fed unpasteurized milk from virus positive lactating cows. Five Holstein calves of approximately 7 to 11 weeks old were fed 0.95 L of unpasteurized milk twice a day via bucket. Milk was collected from two lactating Holstein cows inoculated by an intramammary route and fed to four calves for four days. Milk from two non-inoculated cows was fed to one negative control calf for four days. After four days all five calves were fed milk from non-inoculated cows. Calves fed infected milk showed clinical signs including nasal discharge, mild fever, mild lethargy, loose stool and slightly increased respiratory effort for 5-6 days. Signs of clinical disease were mild and may not be recognized under field conditions with other environmental or health stressors. Viral RNA was consistently detected in nasal swabs from all four calves from 2 to 4 DPI, with consistent detection persisting after calves were transitioned to being fed milk from non-inoculated cows. Infection was confirmed by viral RNA detected in nasal swabs, antigen and viral RNA detection in lung lesions, lymph nodes and pharyngeal tonsil, and seroconversion. Ct values of the nasal swabs were significantly correlated (-0.55) with lethargy in the calves. As Ct values dropped, and viral load increased, calves became more lethargic. Milk diverted from the human food supply in H5N1 positive dairy herds or from suspect cows should not be fed to calves without pasteurization. The determination of routes of transmission like the study described here is an essential first step to inform subsequent research on intervention and vaccination strategies.