Xiang Li, etc.,al. Quick detection of H5N1 avian influenza virus by surface enhanced Raman scattering(SERS) using aptamer capture. Chin J Vet Sci Oct, 2023 Vol.43 No.10
A rapid detection method for H5N1 avian influenza virus was established based on the combination of SERS(surface enhanced Raman scattering)and aptamer capture.Two aptamers with good specificity and sensitivity for the H5N1 avian influenza virus were obtained through screening in the laboratory, acapture reagent for the H5N1 avian influenza virus was established through coupling with magnetic beads, the H5N1 avian influenza virus in a trace amount could be captured, a nano silver particle enhanced Raman signal was generated through in situ reduction on a capture system, and an in situ,rapid,accurate and non destructive Raman spectrum rapid detection technology for the H5N1 avian influenza virus was established. The results showed that the H5N1 avian influenza virus had a strong and symmetrical Raman peak spectrum at 1058cm-1,
and the method had good specificity, stability and sensitivity.The LOD of this method is 1010times the dilution of the proviral solution. The method can quickly detect the presence of H5N1 subtype avian influenza virus in the test sample within 10 minutes and quickly detect a target pathogen in a large volume and low concentration sample, it meets the requirement of on-the-spot quick detection
of the H5N1 avian influenza virus.
and the method had good specificity, stability and sensitivity.The LOD of this method is 1010times the dilution of the proviral solution. The method can quickly detect the presence of H5N1 subtype avian influenza virus in the test sample within 10 minutes and quickly detect a target pathogen in a large volume and low concentration sample, it meets the requirement of on-the-spot quick detection
of the H5N1 avian influenza virus.
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