ObjectiveTo understand the pollution status of avian influenza virus in environment in Yangzhou city during 2012-2020, so as to provide the basis for the prevention and control of avian influenza in Yangzhou city.
MethodsThe environmental samples in Yangzhou from 2012 to 2020 were collected to detect nucleic acid of avian influenza virus by real-time fluorescent PCR. Excel 2016 and SPSS 22.0 software were used for data summary and statistical analysis.
ResultsA total of 270 FluA positive specimens were detected from 3 537 environmental samples, with an overall positive rate of 7.63%. The number of positive samples of H5, H7, H9, and mixed infection were 14, 3, 158 and 95, respectively. There were statistically significant differences in the positive rates in different years (x2=37.06, P<0.01), different months (x2=97.80, P<0.01), different regions (x2=52.36, P<0.01), different monitoring sites (x2=330.67, P<0.01) and in different specimen types (x2=371.16, P<0.01 ).
ConclusionsThere existed avian influenza virus contamination in environment in Yangzhou and H9 subtype and mixed contamination were predominant. The risks were mainly winter and spring, and live poultry markets in urban and rural areas.