Giurgea LT, Park JK, Walters KA, Scherler K, Cerva. The effect of calcium and magnesium on activity, immunogenicity, and efficacy of a recombinant N1/N2 neuraminidase vaccine. NPJ Vaccines. 2021 Apr 6;6(1):48
Despite the importance of immunity against neuraminidase (NA), NA content and immunogenicity are neglected in current influenza vaccines. To address this, a recombinant N1/N2 NA vaccine (NAV) was developed. Stability assays were used to determine optimal temperature and buffer conditions for vaccine storage. The effect of divalent cation-related enhancement of NA stability and activity on N1 and N2 immunogenicity and efficacy against viral challenge was assessed. Differences in activity between N1 and N2 and cation-related activity enhancement did not translate into differences in immunogenicity or efficacy. NAV-vaccinated mice showed robust antibody titers against N1 and N2, and after challenge with influenza A (H1N1) virus, decreased viral titers and decreased antiviral and inflammatory responses by transcriptomic analysis. These findings provide guidance for optimal storage and assessment of NA-based vaccines and confirm the importance of NA in influenza vaccination strategies in attenuating viral replication and limiting inflammatory responses necessary to clear infection.
See Also:
Latest articles in those days:
- Assessment of exposure to influenza A viruses in pigs between weaning and market age 2 hours ago
- Virus Neutralization by Human Intravenous Immunoglobulin Against Influenza Virus Subtypes A/H5 and A/H7 1 days ago
- Balancing the influenza neuraminidase and hemagglutinin responses by exchanging the vaccine virus backbone 3 days ago
- Characterization of Four Novel H5N6 Avian Influenza Viruses with the Internal Genes from H5N1 and H9N2 Viruses and Experimental Challenge of Chickens Vaccinated with Current Commercially Available H5 3 days ago
- Metabolic shifts modulate lung injury caused by infection with H1N1 influenza A virus 4 days ago
[Go Top] [Close Window]