Liu LQ, Li Z, Jiao M, Lu J, Zhou JF, Li XY, Liu J,. Development and Assessment of Two Highly Pathogenic Avian Influenza (HPAI) H5N6 Candidate Vaccine Viruses for Pandemic Preparedness. Biomed Environ Sci. 2020 Sep 20;33(9):670-679
Methods: In accordance with the World Health Organization (WHO) recommendations, we constructed two reassortant viruses using reverse genetics (RG) technology to match the two different epidemic H5N6 viruses. We performed complete genome sequencing to determine the genetic stability. We assessed the growth ability of the studied viruses in MDCK cells and conducted a hemagglutination inhibition assay to analyze their antigenicity. Pathogenicity attenuation was also evaluated in vitro and in vivo.
Results: The results showed that no mutations occurred in hemagglutinin or neuraminidase, and both CVVs retained their original antigenicity. The replication capacity of the two CVVs reached a level similar to that of A/Puerto Rico/8/34 in MDCK cells. The two CVVs showed low pathogenicity in vitro and in vivo, which are in line with the WHO requirements for CVVs.
Conclusion: We obtained two genetically stable CVVs of HPAI H5N6 with high growth characteristics, which may aid in our preparedness for a potential H5N6 pandemic.
- Different intervention strategies toward live poultry markets against avian influenza A (H7N9) virus: model-based assessment 22 hours ago
- Molecular characteristics of H9N2 influenza viruses isolated from farmed raccoon dogs and arctic foxes in China 22 hours ago
- Surveillance of multiple subtype specific antibodies against avian influenza viruses among egg yolk in wild ducks from northeast China, 2017~2019 22 hours ago
- Host ANP32A mediates the assembly of the influenza virus replicase 3 days ago
- Low Pathogenicity Avian Influenza (H5N2) Viruses, Dominican Republic 3 days ago