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2020-7-11 13:29:49

Chambers TM, Reedy SE. Equine Influenza Culture Methods. Methods Mol Biol. 2020;2123:393-400.
submited by kickingbird at Mar, 16, 2020 9:38 AM from Methods Mol Biol. 2020;2123:393-400.

Equine influenza viruses are cultured in embryonated chicken eggs or in mammalian cells, generally Madin-Darby canine kidney (MDCK) cells, using methods much the same as for other influenza A viruses. Mutations associated with host adaptation occur in both eggs and MDCK cells, but the latter show greater heterogeneity and eggs are the generally preferred host. Both equine-1 H7N7 and equine-2 H3N8 viruses replicate efficiently in 11-day-old eggs, but we find that equine-1 viruses kill the embryos whereas equine-2 viruses do not.

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