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2020-7-8 10:16:43

Neumann G. Influenza Reverse Genetics-Historical Perspective. Cold Spring Harb Perspect Med. 2020 Jan 21.
submited by kickingbird at Jan, 25, 2020 9:12 AM from Cold Spring Harb Perspect Med. 2020 Jan 21.

The generation of wild-type, mutant, and reassortant influenza viruses from viral cDNAs (reverse genetics) is now a basic molecular virology technique in many influenza virus laboratories. Here, I describe the original RNA polymerase I reverse genetics system and the modifications that have been developed in past years. Together, these technologies have made possible many advances in basic and applied influenza virology that would not have been otherwise attainable, including the revival and study of extinct influenza viruses, the rapid characterization of emerging influenza viruses, the generation of conventional influenza vaccines, and the development of novel influenza vaccines.

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