-

nihao guest [ sign in / register ]
2019-9-22 6:35:32


Olguin-Perglione C, et al. Multifocal outbreak of equine influenza in vaccinated horses in Argentina in 2018: Epidemiological aspects and molecular characterisation of the involved virus strains. Equine Vet J. 2019 Sep 8.
submited by kickingbird at Sep, 10, 2019 8:45 AM from Equine Vet J. 2019 Sep 8.

BACKGROUND:
Equine influenza is an important cause of respiratory disease of horses worldwide. The equine influenza virus (EIV) undergoes antigenic drift through the accumulation of amino acid substitutions in the viral proteins, which may lead to vaccine breakdown.
OBJECTIVES:
To describe the epidemiological findings and the molecular characteristics of the EIV detected during the multifocal outbreak that occurred in Argentina between March and July 2018 and evidence a vaccine breakdown.
STUDY DESIGN:
Observational, descriptive study.
METHODS:
Virus was detected in nasopharyngeal swabs by real-time RT-PCR. Nucleotide and deduced amino acid sequences of the haemagglutinin (HA) and neuraminidase (NA) genes were obtained from EIV positive nasopharyngeal swabs, and phylogenetic analysis was undertaken. Amino acid sequences were compared against the current World Organisation for Animal Health (OIE)-recommended Florida clade 1 vaccine strain and strain components of vaccines used in Argentina. Serum samples were tested by haemagglutination inhibition test.
RESULTS:
EIV infection was confirmed by real-time RT-PCR and serological testing. The phylogenetic analysis of the HA and NA genes revealed all the EIV identified during the outbreak belong to the H3N8 subtype, Florida clade 1. Multiple amino acid changes, some of them at antigenic sites, were observed in the currently circulating virus when compared with the strains included in the most commonly used vaccine in Argentina. Seventy-six percent of the affected horses had been vaccinated with this vaccine, suggesting the occurrence of vaccine breakdown.
MAIN LIMITATIONS:
The study does not include antigenic characterisation and full genome sequencing of Argentinian strains, that could provide additional information.

See Also:

Latest articles in those days:

[Go Top]    [Close Window]

Related Pages:
Learn about the flu news, articles, events and more
Subscribe to the weekly F.I.C newsletter!


  

Site map  |   Contact us  |  Term of use  |  FAQs
Copyright ©www.flu.org.cn. 2004-2019. All Rights Reserved. Powered by FIC 4.0.1
  Email:webmaster@flu.org.cn