Yamagata Y, et al. Generation of a purely clonal defective interfering influenza virus. Microbiol Immunol. 2019 Apr 18.
A defective interfering (DI) influenza virus carries a large deletion in a viral gene segment and shows potential for antiviral therapy by interfering with the replication of infectious virus. However, because a DI virus cannot replicate autonomously without the aid of an infectious helper virus, clonal DI virus stocks without contamination of the helper virus have not been generated. To overcome this problem, we generated a clonal DI virus with a PB2 DI gene by reverse genetics, amplified the clonal DI virus using a cell line stably expressing the PB2 protein, and confirmed its ability to interfere with infectious virus replication in vitro. Thus, our approach is suitable for obtaining purely clonal DI viruses, will contribute to the understanding of DI virus interference mechanisms, and can be used for the development of DI virus-based antivirals.
See Also:
Latest articles in those days:
- Abundant Intra-Subtype Reassortment Revealed in H13N8 Influenza Viruses 21 hours ago
- Locations and structures of influenza A virus packaging-associated signals and other functional elements via an in silico pipeline for predicting constrained features in RNA viruses 1 days ago
- Molecular Characterization of Non-H5 and Non-H7 Avian Influenza Viruses from Non-Mallard Migratory Waterbirds of the North American Flyways, 2006~2011 1 days ago
- A case report and literature review on tocilizumab-cured acute necrotizing encephalopathy caused by influenza A virus 1 days ago
- Study on the clinical efficacy and safety of baloxavir marboxil tablets in the treatment of influenza A 1 days ago
[Go Top] [Close Window]