Amarelle L, et al. Cardiac glycosides decrease influenza virus replication by inhibiting cell protein translational machinery. Am J Physiol Lung Cell Mol Physiol. 2019 Mar 20
Cardiac glycosides (CG) are used primarily for cardiac failure and have been reported to have other effects, including inhibition of viral replication. Here we set out to study mechanisms by which CG as inhibitors of the Na,K-ATPase, decrease influenza A virus (IAV) replication in the lungs. We found that CG inhibit influenza virus replication in alveolar epithelial cells by decreasing intracellular potassium which in turn inhibits protein translation, independently of viral entry, mRNA transcription, and protein degradation. These effects were independent of the Src signaling pathway and intracellular calcium concentration changes. We found that short-term treatment with ouabain prevented IAV replication without cytotoxicity. Rodents express a Na,K-ATPase α1 resistant to GC. Thus, we utilized Na,K-ATPase α1 sensitive mice, infected them with high doses of influenza virus and observed a modest survival benefit when treated with ouabain. In summary, we provide evidence that the inhibition of the Na,K-ATPase by CG decreases influenza A viral replication by modulating the cell protein translational machinery and results in a modest survival benefit in mice.
See Also:
Latest articles in those days:
- Epidemiological characteristics of human infections with avian influenza A(H5N6) virus, China and Laos: A multiple case descriptive analysis, February 2014-June 2023 10 hours ago
- Interim Estimates of 2023-2024 Seasonal Influenza Vaccine Effectiveness Among Adults in Korea 20 hours ago
- Abundant Intra-Subtype Reassortment Revealed in H13N8 Influenza Viruses 2 days ago
- Locations and structures of influenza A virus packaging-associated signals and other functional elements via an in silico pipeline for predicting constrained features in RNA viruses 2 days ago
- Molecular Characterization of Non-H5 and Non-H7 Avian Influenza Viruses from Non-Mallard Migratory Waterbirds of the North American Flyways, 2006~2011 2 days ago
[Go Top] [Close Window]