Hong ZY, et al. Equipping inner central components of influenza A virus with quantum dots. Anal Chem. 2018 Nov 5.
Influenza A virus (IAV), a risk to public health, is enveloped and contains viral ribonucleoprotein (vRNP) complexes, where vRNP complexes are centrality to every aspect of IAV life cycle. Labeling both the vRNP complexes and viral envelope with quantum dots (QDs) is conducive to achieving globally long-term tracking of single IAV infecting host cell, which has potential to provide valuable information for revealing mechanisms of IAV infection. However, even though some strategies for labeling of viral envelope with QDs have been developed, there are few strategies for coupling of QDs to the vRNP complexes inside IAV so far. Herein, we devised a convenient electroporation based strategy, coupled with antibody binding, to transfer green QDs labeled nucleoprotein antibodies (GQDs-NPAb) into H1N1 and achieved the labeling of vRNP complexes with QDs (H1N1(GQDs)). Under optimal condition of 20 nM GQDs-NPAb and a single pulse with 20 ms duration and 750 V/cm pulse intensity, the actual efficiency of labeling is ca. 34% and H1N1(GQDs) can retain 93% infectivity. Then, dual-labeling of H1N1 was realized by labeling the envelope of H1N1(GQDs) with red QDs (RQDs) via a mild and efficient hydrazine-aldehyde based strategy. At the optimal RQDs concentration of 5 nM, the actual efficiency of dual-labeling can reach to 11% and the dual-labeled H1N1 can retain 93% infectivity. Because of the similar components and structure of different IAV subtypes, this dual-labeling strategy is applicable to other subtypes of IAV, e.g. H9N2.
See Also:
Latest articles in those days:
- Phylogeography and gene pool analysis of highly pathogenic avian influenza H5N1 viruses reported in India from 2006 to 2021 20 hours ago
- Analysis of a diffusive epidemic model with a zero-infection zone 21 hours ago
- Quick detection of H5N1 avian influenza virus by surface enhanced Raman scattering(SERS) using aptamer capture 21 hours ago
- The critical role of RAGE in severe influenza infection: A target for control of inflammatory response in the disease 21 hours ago
- Human infection caused by avian influenza A (H10N5) virus 22 hours ago
[Go Top] [Close Window]