-

nihao guest [ sign in / register ]
2024-3-29 2:31:15


Liu J, Gong LF, Xu YC, Sun ZL, Gao Q, Dong ZJ. Genetic and antigenic characterization of influenza A(H1N1)pdm09 in Yantai, China, during the 2009-2017 influenza season. J Med Virol. 2018 Sep 29.
submited by kickingbird at Oct, 3, 2018 19:26 PM from J Med Virol. 2018 Sep 29.

OBJECTIVE:
This study was performed to determine the antigenic and genetic characteristics and evaluate potential vaccine efficacy of influenza A(H1N1)pdm09 in Yantai from August 2009 to August 2017.
MATERIALS AND METHODS:
A total of 10236 swabs were collected among patients with an influenza-like illness (ILI) who were admitted to 2 sentinel surveillance hospitals in Yantai, East China, from August 2009 to August 2017. All specimens were cultured in Madin-Darby canine kidney cells and identified by haemagglutination inhibition assay. Complete sequences of Haemagglutinin (HA) and Neuraminidase (NA) of fifty-one influenza A(H1N1)pdm09 strains circulating in Yantai were amplified, sequenced and analysed using Molecular and phylogenetic methods. The potential vaccine efficacy was calculated using the p epitope model which measured the antigenic variation based on the changes in the dominant epitope of HA.
RESULTS:
The results showed that most Yantai strains were grouped into genetic clades 1.7, 6C, 6B.1 and 6B.2. The amino acid substitutions accumulated gradually in HA proteins and considerable genetic variation were oberserved in circulating A(H1N1)pdm09 viruses during the seven influenza seasons. The V241I, N369K, N386K and K432E mutations which may change the binding pattern and affinity of oseltamivir for neuraminidase were detected in the strains circulating in 2016/2017 and 2017/2018 seasons and the recommended vaccine strains could afford optimal protection against the influenza A/H1N1pdm09.
CONCLUSIONS:
Although influenza A(H1N1)pdm09 viruses acquired significant genetic variation over the course of 7 influenza seasons, the recommended vaccine strains still afforded protection against main circulating strains. Continuous epidemiological and virological surveillance are necessary.

See Also:

Latest articles in those days:

[Go Top]    [Close Window]

Related Pages:
Learn about the flu news, articles, events and more
Subscribe to the weekly F.I.C newsletter!


  

Site map  |   Contact us  |  Term of use  |  FAQs |  粤ICP备10094839号-1
Copyright ©www.flu.org.cn. 2004-2024. All Rights Reserved. Powered by FIC 4.0.1
  Email:webmaster@flu.org.cn