OIE: Highly pathogenic avian influenza ,Russia

Information received on 27/04/2015 from Dr Evgeny Nepoklonov, Deputy Head, Federal Service for Veterinary and Phytosanitary Surveillance, Ministry of Agriculture, Moscow, Russia

Summary

Report type	Immediate notification (Final report)
Date of start of the event	17/04/2015
Date of confirmation of the event	21/04/2015
Report date	27/04/2015
Date submitted to OIE	27/04/2015
Date event resolved	27/04/2015
Reason for notification	Reoccurrence of a listed disease
Date of previous occurrence	10/2014
Manifestation of disease	Sub-clinical infection
Causal agent	Highly pathogenic avian influenza virus
Serotype	H5N1
Nature of diagnosis	Laboratory (basic), Laboratory (advanced)
This event pertains to	a defined zone within the country

New outbreaks

Summary of outbreaks

Total outbreaks: 1

Outbreak Location

ASTRAKHANSKAYA OBLAST ( Makarkin, Ikryaninsky )

Total animals affected

Species	Susceptible	Cases	Deaths	Destroyed	Slaughtered
Dalmatian pelican:Pelecanidae (Pelecanus crispus)		5	5	0	0

Outbreak statistics

Species	Apparent morbidity rate	Apparent mortality rate	Apparent case fatality rate	Proportion susceptible animals lost*
Dalmatian pelican:Pelecanidae (Pelecanus crispus)	**	**	100.00%	**

* Removed from the susceptible population through death, destruction and/or slaughter;
** Not calculated because of missing information;

Epidemiology

Source of the outbreak(s) or origin of infection

Unknown or inconclusive

Epidemiological comments

The comparative analysis of the obtained nucleotide sequences of the H gene 258 bp fragment showed that the tested isolates belonged to an Asian lineage of highly pathogenic avian influenza (lineage A/Guandong/1/96 lineage, clade 2.3.2.1).
Based on the H gene fragment nucleotide sequences the given isolates were the most similar to type A H5N1 highly pathogenic avian influenza virus isolates recovered in Altay krai in 2014 (98.83 % identity) and in Vietnam and China over the period from 2012 to 2014 (98.44-98.04% identity).
The hemagglutinin cleavage site of this virus includes several basic amino acids and has -RERRRKR- structure which allows this virus to be characterized as potentially highly virulent.

Control measures

Measures applied	Control of wildlife reservoirs Screening No vaccination No treatment of affected animals
Measures to be applied	No other measures

Diagnostic test results

Laboratory name and type

Government Budget Institution Astrakhan Oblast Veterinary Laboratory ( Local laboratory )

Tests and results

Species	Test	Test date	Result
Dalmatian pelican	polymerase chain reaction (PCR)	21/04/2015	Positive

Laboratory name and type

All-Russian Research Institute for Animal Health (FGBI-ARRIAH) ( National laboratory )

Tests and results

Species	Test	Test date	Result
Dalmatian pelican	gene sequencing	24/04/2015	Positive
Dalmatian pelican	haemagglutination inhibition test (HIT)	24/04/2015	Positive
Dalmatian pelican	real-time reverse transcriptase/polymerase chain reaction (RRT-PCR)	24/04/2015	Positive
Dalmatian pelican	virus isolation	24/04/2015	Positive

Future Reporting

The event is resolved. No more reports will be submitted.