We detected three avian influenza HA subtypes (H7, H9 and H5) and two NA subtypes (N9 and N2), as well as H7N9-related reassortant intermediates H9N9, co-circulating among poultry in Huzhou, China, during April 2013. The co-circulation not only reveals that Huzhou is one of the geographic origins of the novel H7N9 virus, but also poses a potential threat to humans in the future.