Whole genome sequencing and analysis of three strains of Avian influenza virus subtype H9N2 isolated in Guangxi province

Eight pairs of specific primers were designed and synthesized according to the sequences of Avian influenza virus(AIV) subtype H9N2 released in GenBank,and they were used in RT-PCR.In this way,3 isolates of AIV subtype H9N2 were obtained namely,A/chicken/Guangxi/1/00,A/chicken/Guangxi/14/00 and A/chicken;Guangxi/17/00 and their complete nucleotide sequences were analysed and compared.It was found that as demonstrated by homology and phylogenic analysis,higher homologues in the corresponding nucleotide seque...

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