We isolated and characterized three H5 HPAIVs with different NA subtypes (N1, N6 and N8) during avian influenza virus (AIV) surveillance in wild birds during 2015-2016. The P126/H5N1, P560/H5N6 and ST/H5N8 viruses were highly pathogenic to chickens and ducks. Furthermore, P126/H5N1 and P560/H5N6 showed high pathogenicity in mice. Continued circulation of these influenza virus strains clearly poses a significant potential health threat to poultry and human populations.